Synthesis of 2,4-pyrimidinediamines

ABSTRACT

Disclosed herein are methods for synthesizing 2,4-pyrimidinediamines as well as intermediates used therein.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. application Ser. No.13/625,552, filed Sep. 24, 2012, which is a divisional of U.S.application Ser. No. 12/829,126, filed Jul. 1, 2010 now U.S. Pat. No.8,299,242, issued Oct. 30, 2012, which claims the benefit of U.S.Provisional Application Ser. No. 61/270,073, filed Jul. 2, 2009, whichare hereby incorporated by reference in their entirety.

I. BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates to the field of pharmaceutical/process chemistry.Disclosed herein are methods for synthesizing 2,4-pyrimidinediamines aswell as intermediates used therein. As an embodiment, provided herein isa process for preparing N4-(2,2-dimethyl-4-[(dihydrogenphosphonoxy)methyl]-3-oxo-5-pyrido[1,4]oxazin-6-yl)-5-fluoro-N2-(3,4,5-trimethoxyphenyl)-2,4-pyrimidinediaminedisodium salt (compound of formula I), particularly hydrates (such as ahexahydrate) of the compound of formula I, a 2,4-pyrimidinediamine thatis useful in the treatment and prevention of various diseases.

2. Summary of the Related Art

Various-classes of 2,4-pyrimidinediamine compounds have been discoveredthat have myriad therapeutic uses. See, for example, U.S. applicationSer. No. 10/355,543 filed Jan. 31, 2003 (U.S. 2004/0029902A1),international application Ser. No. PCT/U.S.03/03022 filed Jan. 31, 2003(WO 03/063794), U.S. application Ser. No. 10/631,029 filed Jul. 29, 2003(U.S.2007/0060603), international application Ser. No. PCT/U.S.03/24087(WO 2004/014382), U.S. application Ser. No. 10/903,263 filed Jul. 30,2004 (U.S.2005/0234049), and international application Ser. No.PCT/U.S.2004/24716 (WO/2005/016893). Each of these applications isincorporated by reference in its entirety.

One of the process for preparing the 2,4-pyrimidinediamine compounds isdescribed in U.S. application Ser. No. 11/539,074, filed Oct. 5, 2006,which is incorporated herein by reference in its entirety in the presentdisclosure.

II. SUMMARY OF THE INVENTION

The invention comprises a processes for preparingN4-(2,2-dimethyl-4-[(dihydrogenphosphonoxy)methyl]-3-oxo-5-pyrido[1,4]oxazin-6-yl)-5-fluoro-N2-(3,4,5-trimethoxyphenyl)-2,4-pyrimidinediaminedisodium salt (compound of formula I):

as well as hydrates (such as a hexahydrate) thereof. The compound offormula I (and hydrates thereof) is a 2,4-pyrimidinediamine that isuseful in the treatment and prevention of various diseases. Theinvention further comprises solvate intermediates useful in the processas well as the compound produced by the process.

It will be appreciated by one of skill in the art that the embodimentssummarized above may be used together in any suitable combination togenerate embodiments not expressly recited above and that suchembodiments are considered to be part of the present invention.

III. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 illustrates a dynamic differential scanning calorimetryexperiment (DSC) in closed cup of neat di-tert-butylchloromethylphosphate (3.834 mg).

FIG. 2 illustrates a Thermo-Graphic-Analysis (TGA) experiment with neatdi-tert-butylchloromethyl phosphate (7.7 mg).

FIG. 3 illustrates a dynamic differential scanning calorimetryexperiment in closed cup of a 36% solution of di-tert-butylchloromethylphosphate (5.6 mg) in DMAc.

FIG. 4 illustrates a isotherm differential scanning calorimetryexperiment in closed cup of a 36% di-tert-butylchloromethyl phosphate(10.9 mg) solution in DMAc at 80° C.

IV. DETAILED DESCRIPTION OF THE INVENTION 1. Definitions

As used herein, the following definitions shall apply unless otherwiseindicated.

Unless defined otherwise, all technical, and scientific terms usedherein have the same meanings as commonly understood by one of ordinaryskill in the art to which this invention belongs. Although any methodsand materials similar or equivalent to those described herein can beused in the practice or testing of the present invention, the preferredmethods, devices, and materials are now described. All publicationscited herein are incorporated herein by reference in their entirety forthe purpose of describing and disclosing the methodologies, reagents,and tools reported in the publications that might be used in connectionwith the invention. Nothing herein is to be construed as an admissionthat the invention is not entitled to antedate such disclosure by virtueof prior invention.

“Amide” refers to the group R²⁰CON(R²¹)₂ wherein R²⁰ is selected fromhydrogen or optionally substituted alkyl; and each R²¹ is independentlyhydrogen or optionally substituted alkyl, or both of R²¹ and thenitrogen with which they are attached form a 4 to 6 membered aliphaticring; or R²⁰ and one of the R²¹ join together with the carbon andnitrogen to which they are attached, respectively, combine to form a 4to 6 membered nitrogen containing ring, and the other R²¹ is hydrogen oroptionally substituted alkyl. Amides include primary amides, secondaryamides (such as, but not limited to, alkyl formamides and acetamides,such as N-methyl acetamide), and tertiary amides (such as, but notlimited to, N,N-dialkylacetamides, N,N-dialkylformamides,N-alkylpyrrolidones, and N-alkylpiperidones). Particular examples oftertiary amides suitable for use in the presently disclosed solvatesinclude, without limitation N,N-dimethylacetamide,N,N-dimethylformamide, N-methylpyrrolidinone, N-methylpiperidinone.

“Alkyl” refers to monovalent saturated aliphatic hydrocarbyl groupshaving from 1 to 8 carbon atoms, such as, 1 to 6 carbon atoms or 1 to 4carbon atoms. This term includes, by way of example, linear and branchedhydrocarbyl groups such as methyl (CH₃—), ethyl (CH₃CH₂—), n-propyl(CH₃CH₂CH₂—), isopropyl ((CH₃)₂CH—), n-butyl (CH₃CH₂CH₂CH₂—), isobutyl((CH₃)₂CHCH₂—), sec-butyl ((CH₃)(CH₃CH₂)CH—), t-butyl ((CH₃)₃C—),n-pentyl (CH₃CH₂CH₂CH₂CH₂—), and neopentyl ((CH₃)₃CCH₂—). Also by way ofexample, a methyl group, an ethyl group, an n-propyl, an isopropylgroup, a n-butyl group, an isobutyl group, sec-butyl group, and t-butylare all represented by the term C₁-C₄ alkyl. Likewise terms indicatinglarger numerical ranges of carbon atoms are representative of any linearor branched hydrocarbyl falling within the numerical range. Thisinclusiveness applies to other hydrocarbyl terms bearing such numericalranges.

“Base” refers to substance that can accept protons. Examples of basesinclude, but are not limited to, carbonates, such as cesium carbonate,sodium carbonate, sodium bicarbonate, potassium carbonate, hydroxides,such as, sodium hydroxide, potassium hydroxide, lithium hydroxide, andammonia.

“Halo” or “halogen” refers to fluoro, chloro, bromo, and iodo.

“Solvate” refers to a complex formed by combination of at least onesolvent molecule with at least one molecule or ion of the solute. One ofordinary skill in the art will appreciate that the stoichiometry of thesolvent to the solute in a solvated may be greater than one, equal toone or less than one. The solvent can be an organic compound, aninorganic compound, or a mixture of both. Some examples of solventsinclude, but are not limited to, methanol, acetic acid,N,N-dimethylformamide, tetrahydrofuran, dimethylsulfoxide, and water.When used herein, the term “solvate” is not intended to restrict thesolvate compounds described herein to any particular sort of bonding(such as ionic or coordinate covalent bonds).

The term “substituted,” when used to modify a specified group orradical, means that one or more hydrogen atoms of the specified group orradical are each, independently of one another, replaced with the sameor different substituent groups as defined below.

Substituent groups on optionally substituted alkyls are alkyl, halo,haloalkyl, nitroso, and cyano.

Similarly, it is understood that the above definitions are not intendedto include impermissible substitution patterns (such as carbonsubstituted with five groups, i.e., pentavalent carbon). Suchimpermissible substitution patterns are easily recognized by a personhaving ordinary skill in the art.

2. Compositions and Process

Disclosed herein are methods for synthesizing 2,4-pyrimidinediamines aswell as intermediates used therein. As an embodiment, provided herein isa process for preparing N4-(2,2-dimethyl-4-[(dihydrogenphosphonoxy)methyl]-3-oxo-5-pyrido[1,4]oxazin-6-yl)-5-fluoro-N2-(3,4,5-trimethoxyphenyl)-2,4-pyrimidinediaminedisodium salt (Compound of formula I) (including hydrates thereof,particularly a hexahydrate), a 2,4-pyrimidinediamine that is useful inthe treatment and prevention of various diseases.

The compound of formula I has been described in U.S. Pat. No. 7,449,458,filed Jan. 19, 2006, which is incorporated herein by reference in itsentirety in the present disclosure. The phosphate containing progroupssuch as in the compounds of formulae I, II, III, and VI may increase thesolubility of the 2,4-pyrimidinediamine compounds that exhibit poorsolubility under physiological conditions (for example, solubilities ofless than about 10 μg/mL). The phosphate-containing progroups may aidthe solubility of the underlying active 2,4-pyrimidinediamine compound,which in turn may increase its bioavailability when administered orally.The phosphate progroups may be metabolized by phosphatase enzymes foundin the digestive tract, permitting uptake of the underlying active drug.

The U.S. Pat. No. 7,449,458, filed Jan. 19, 2006, discloses that thewater solubility and oral bioavailability of a particular biologicallyactive 2,4-pyrimidinediamine compound, such as compound of formula IV:

increased dramatically when formulated to include a progroup at the ringnitrogen atom as in compound of formula II:

Where the water solubility of the compound of formula IV was found to bein the range of about 1-2 μg/mL in aqueous buffer under physiologicalconditions, the solubility of the corresponding phosphate prodrug(compound of formula II) was found to be greater than 5 mg/mL under thesame conditions, or approximately 2000 times greater. This increasedwater-solubility allows for better dissolution in the gut, therebyfacilitating oral administration.

A process for preparing the compound of formula I has been described inU.S. application Ser. No. 11/539,074, filed Oct. 5, 2006, which isincorporated herein by reference in its entirety in the presentdisclosure.

In a broad aspect, the invention is directed to a process for making anamide solvate of a compound of formula II (below) (formula IIa) andconverting it into the compound of formula I. In a more specific sense,the invention is directed to a process where an acid solvate of thecompound of formula II is converted to an amide solvate, and the amidesolvate converted to the compound of formula I. In a more specificembodiment, the invention is directed to a process for preparing acompound of formula I:

comprising:

a) contacting an acid solvate of a compound of formula II:

-   -   with an amide under conditions suitable for forming an amide        solvate of the compound of formula II; and

b) contacting the amide solvate with an aqueous base comprising sodiumions under conditions suitable for forming the compound of formula I. Ina particular embodiment, the compound of formula I is a hydrate, such asa hexahydrate.

In some embodiments, the acid solvate of the compound of formula II is acarboxylic acid. In some embodiments, the carboxylic acid is R¹COOHwherein R¹ is —H or a C₁-C₄ alkyl optionally substituted with up tothree halo substituents.

In another aspect the invention comprises the novel amide solvateintermediates used in the processes described herein. For example, thedisclosed compounds include an amide solvate of formula IIa:

With reference to formula IIa, the amide solvate is not limited to themonosolvate, but may include solvates of multiple and non-integernumbers of amide molecules per molecule of compound II, such as, 0.5, 1,2, and 3.

In some embodiments, the amide is a secondary amide or a tertiary amide.

In some embodiments, the amide is R³⁰CON(R²)₂ where each R² isindependently —H or C₁-C₄ alkyl or both R² together with the nitrogen towhich they are attached form a 4 to 6 membered aliphatic ring, and R³⁰is —H or C₁-C₄ alkyl; or R³⁰ and one of the R² together with the carbonand nitrogen to which they are attached, respectively, combine to form a4 to 6-membered aliphatic ring and the other R² is independently —H orC₁-C₄ alkyl. In some embodiments, the amide is selected from the groupconsisting of a N,N-dialkylformamide, N,N-dialkylacetamide,N-alkylpyrrolidinone and N-alkylpiperidone.

In some embodiments, the amide is selected from the group consisting ofa N,N-dialkylformamide, N,N-dialkylacetamide, N-alkylpyrrolidinone andN-alkylpiperidone. In some embodiments, the amide isN,N-dimethylformamide (DMF):

The amide solvate IIa can be synthesized by conversion of an acidsolvate of the compound for formula II to the amide solvate IIa. One ofordinary skill in the art will recognize in view of the presentdisclosure that the amide solvates of formula IIa can be made via otherforms of II, not only acid solvates of II. In some embodiments, theamide is the N,N-dialkylformamide and the conditions suitable forforming the amide solvate of the compound of formula II comprisecontacting the acid solvate with the tertiary amide at a temperature ofbetween about 20° C. and about 50° C. In some embodiments, the amide isN,N-dimethylformamide (DMF) and the conditions suitable for forming theamide solvate comprise re-slurrying the acid solvate in the DMF at atemperature of about 40° C.

In some embodiments, the aqueous base in step b) above comprises sodiumhydroxide (NaOH) and an alcohol, and the conditions suitable for formingthe compound of formula I comprise a temperature of between about 40° C.about 80° C. and a pH of about 9 to about 10.5. In some embodiments, thealcohol includes, but is not limited to, methanol, ethanol,iso-propanol, butanol, t-butanol, pentanol.

In some embodiments, the aqueous base in step b) above comprises sodiumhydroxide (NaOH) and isopropyl alcohol (IPA), and the conditionssuitable for forming the compound of formula I comprise a temperature ofabout 80° C. and a pH of about 10.2.

In another aspect, the invention comprises a process for preparing aprecursor (VI) of the compound of formula I:

comprising contacting a compound of formula IV:

with a compound of formula V:

in the presence of an amide,

wherein:

-   -   R³ and R⁴ are each independently C₁-C₆ alkyl; and    -   X is halogen or —OSO₂R⁶², where R⁶² is alkyl optionally        substituted with halogen (e.g., perfluorinated alkyl groups) or        aryl optionally substituted with alkyl or an electron        withdrawing group, e.g. halogen, NO₂, —CN and the like        (preferably X is halogen);

under conditions suitable for forming a compound of formula VI:

In another embodiment, the invention comprises a method of convertingthe precursor VI into the compound of formula I, the method comprising:

-   -   a) contacting the compound of formula VI with an acid under        conditions suitable for forming an acid solvate of a compound of        formula II:

-   -   b) contacting the acid solvate of the compound of formula II        with an amide under conditions suitable for forming an amide        solvate of the compound of formula II; and    -   c) contacting the amide solvate of the compound of formula II        with an aqueous base comprising sodium ions under conditions        suitable for forming the compound of formula I.        In a particular embodiment, the compound of formula I produced        by this method is a hydrate, such as a hexahydrate.

In another embodiment, the invention comprises the sequentialcombination of the two previous methods (i.e., a method comprising themethod of making precursor VI followed by the method of converting VI tothe compound of formula I).

In a more generic sense, the invention is directed to making thecompound of formula I by: reacting compounds IV and V to give compoundVI; converting compound VI to compound II, or an acid solvate of II;converting compound II, and/or its acid solvate, to compound IIa, theamide solvate; and converting compound IIa to compound I (optionally inthe form of a hydrate, such as a hexahydrate).

In some embodiments, the compound of formula V is di-tert-butylchloromethyl phosphate:

The conditions suitable for producing the compound of formula VI cancomprise:

-   -   (i) combining the compound of formula IV with the compound of        formula V with a base in a polar solvent; and    -   (ii) washing the product obtained from step (i) in an aqueous        base solution.

Examples of bases suitable for use in steps (i), (ii) or both include,but are not limited to, carbonates, such as cesium carbonate, sodiumcarbonate, sodium bicarbonate, potassium carbonate, hydroxides, such as,sodium hydroxide, potassium hydroxide, lithium hydroxide, and 1°, 2° and3° amines such as triethylamine, N,N-dimethylaniline, N,N-diethylanilineand ammonia, as well as metal alkoxides e.g. potassium t-butoxide.

Examples of polar solvent include, but are not limited to,N,N-dimethylformamide (DMF), N,N-dimethylacetamide, tetrahydrofuran,dichloromethane, acetone, acetonitrile, dimethylsulfoxide. Mixtures ofsuch solvents also can be used as is known to those of skill in the art.Moreover, those of skill in the art also would understand that suchpolar solvents can include non-polar components in a mixture with one ormore polar solvents so long as the resultant solvent mixture is polar.Solvents typically considered to be polar include those having adielectric constant, E, of at least about 5 and typically greater thanabout 7 or 8. For example, tetrahydrofuran has a dielectric constant, Eof 7.6, whereas DMF has a dielectric constant of 37.

In some embodiments, the base in step (i) above comprises at least oneof cesium carbonate (Cs₂CO₃) and potassium carbonate (K₂CO₃); the polarsolvent comprises at least one of DMF and N,N-dimethylacetamidc (DMAc);and the aqueous base in step (ii) above comprises at least one of sodiumbicarbonate (NaHCO₃) and sodium hydroxide (NaOH).

In some embodiments, the compound of formula VI is not isolated.

In some embodiments, the compound of formula V is stabilized withN,N-dimethylacetamide (DMAc) solvent.

Generally, the compound of formula V, such as, di-tert-butylchloromethyl phosphate, is an unstable product. As an example,di-tert-butyl chloromethyl phosphate undergoes decomposition uponstorage to give off heat and iso-butene gas. With reference to thescheme below, and while not intending to be bound by theory, it isbelieved that the presence of trace amounts of acid catalyzes thecleavage of an O-tert-butyl group on di-tert-butyl chloromethylphosphate A to give mono-text-butyl species C with the release ofisobutene. Species C can act as an acid source further drivingautocatalytic decomposition to phosphate E. As indicated by the dottedline in the scheme below, phosphate E can also provide protons to feedinto the autocatalytic decomposition of A. Decomposition of A isexothermic and produces two moles of isobutene per mole of A.

When stored under adiabatic conditions, the heat and pressure build-upfrom decomposition can be significant. FIG. 1 illustrates that storageof di-tert-butyl chloromethyl phosphate under adiabatic conditions canresult in decomposition whereby the pressure and temperature increasedramatically. FIG. 1 illustrates a dynamic differential scanningcalorimetry experiment (DSC) in closed cup of neatdi-tert-butylchloromethyl phosphate (heating from 0° C. to 300° C. at arate of 5° C./min under a N₂ flow of 50 mL/min.). Referring to FIG. 1,after an endothermic signal (start of isobutene release under uponformation of acidic by products; extrapolated peak at 99.10° C. with apeak width of 0.33° C. and an integrated area of −108.45 mJ) a verysharp exothermic signal at about 100° C. is observable (extrapolatedpeak at 100.57° C. with a peak width of 3.99° C. and a integrated areaof 2717.05 mJ), which is typical for an autocatalytic decomposition.FIG. 2 illustrates a Thermo-Graphic-Analysis (TGA) experiment with neatdi-tert-butylchloromethyl phosphate, showing that continuousdecomposition is observed with isobutene offgassing. In FIG. 2, thesample was heated from 20° C. to 300° C. at a rate of 5° C./min under N₂at a flow rate of 80 mL/min. The sample showed a 41.465% (3.189 mg) massloss between about 21° C. and 119° C.; and a 19.526% (1.502 mg) massloss between about 119° C. and 300° C. Most of the isobutene is sharplysplit off at 110° C.; after 1 h at 300° C. the weight of the samplecorresponds to acid E as depicted above. Further tests have shown thepressure increase due to isobutene release can be as great as 80 bar.Also, isobutene is a flammable gas therefore venting large quantities ofiso-butene can be dangerous.

Therefore, it is normally critical to design equipment for the storageof the compound of formula V that can withstand the pressure build up.Various other safety measures need to be taken as well, such astemperature control, distillation time, and safety valve dimension, toavoid equipment damage during the unwanted decomposition reaction.

However, it was unexpectedly found that the addition ofN,N-dimethylacetamide (DMAc) stabilizes di-tert-butyl chloromethylphosphate such that the compound may be stored at about 60° C. with noautocatalytic decomposition and no gas being formed (see FIGS. 3-4).FIG. 3 illustrates a dynamic differential scanning calorimetryexperiment in closed cup of a 36% solution of di-tert-butylchloromethylphosphate in DMAc (heating from 0° C. to 300° C. at a rate of 5° C./min.under a N₂ flow of 50 mL/min.). After an endothermic signal (someisobutene loss due to trace acid; extrapolated peak at 116.42° C. with apeak width of 6.19° C. and an integrated area of −70.78 mJ) a smoothexothermic signal at 120° C. is observable (no sharp exothermic signalat 99 to 100° C. is observable; extrapolated peak at 129.74° C. with apeak width of 42.52° C. and an integrated area of 1362.40 mJ). Thisindicates that the system is not undergoing autocatalytic decomposition.FIG. 4 illustrates an isothermic differential scanning calorimetry (DSC)experiment in closed cup of a 36% di-tert-butylchloromethyl phosphatesolution in DMAc at 80° C. (under a N₂ flow of 50 mL/min.). Noendothermic or exothermic decomposition of a 36%di-tert-butylchloromethyl phosphate solution in DMAc is observed atstorage temperatures (45, 60 and even 80° C.) over 15 hours, thus thedi-tert-butylchloromethyl phosphate is stabilized. In fact, isothermalheating at 60° C. of a solution from 68.7 g of a 36% solution ofdi-tert-butylchloromethyl phosphate in DMAc for over 96 hours generatedno gas (isobutene).

It is to be understood that any amide, such as, but not limited to,DMAc, may be used to stabilize the compound of formula V, includingdi-tert-butyl chloromethyl phosphate. Such amides are well known to aperson of ordinary skill in the art. Examples of such amides include,but are not limited to, N,N-dimethylacetamide, N,N-dimethylformamide,N-methylpyrrolidinone. In some embodiments, a solvent may be optionallyadded to a combination of the amide and di-tert-butyl chloromethylphosphate. In some embodiments, the amide may also be a solvent. Forexample, DMAc can be used as the amide as well as the solvent.

Accordingly, in one aspect, there is provided a composition comprisingdi-tert-butyl chloromethyl phosphate:

and an amide optionally in a solvent.

In some embodiments, the amide is also the solvent.

In some embodiments, the amide is a tertiary amide.

In some embodiments, the tertiary amide is N,N-dimethylacetamide (DMAc).

The improved process for the synthesis of the compound of formula I isas illustrated in Schemes I-VII below.

3. Synthetic Schemes

Starting materials used in the synthesis described herein are availablecommercially. The synthesis of the compound of formula V is as shown inScheme I below:

According to Scheme I, compound of formula V is obtained by the reactionof potassium dialkyl phosphate group (R³ and R⁴ as defined hereinabove)with an alkylating agent (X as defined hereinabove), such as ahalomethylchlorosulfate in the presence of a phase transfer catalyst(PTC). Numerous examples of phase transfer catalysts are known to thoseof skill in the art. Examples of such phase transfer catalysts include,without limitation tetraalkyl ammonium salts, such as tetrabutylammonium salts. For example, di-tert-butyl chloromethyl phosphate can beobtained by the reaction of potassium or sodium di-tert-butyl phosphate(PDP) with chloromethylchlorosulfate (CMCS) in the presence oftetrabutylamonium bisulfate (TBAHS).

Scheme Ia below (as well as FIGS. 1-4 and the Examples below), showsthat the pH adjustment and the addition of N,N-dimethylacctamide (DMAc)stabilizes di-tert-butyl chloromethyl phosphate such that the compoundmay be kept at about 60° C. with no gas being formed.

It is to be understood that the synthesis of di-tert-butyl chloromethylphosphate in Scheme Ia is for illustration purposes only. Synthesis ofother phosphates of the compound of formula V by following Scheme Ia canbe carried out by routine adaptation of the method. In addition, sodiumor other salts of di-tert-butyl phosphate and other reaction conditionscan be used to make di-tert-butyl chloromethyl phosphate.

The synthesis of the compound of formula VIa from compound of formula IVis as illustrated in Scheme II below:

According to Scheme II, the compound of formula IV is treated withdi-tert-butyl chloromethyl phosphate to result in the compound offormula VIa (such as step B of Example 1 herein). It is to be understoodthat reaction of a compound of formula IV with di-tert-butylchloromethyl phosphate is for illustration purposes only. Otherphosphates, such as compound of formula V described herein, may bereacted with the compound of formula IV to result in the compound offormula VI using routine adaptation of the method. The steps describedfurther below for the compound of formula VIa may also be applied to thecompound of formula VI.

In some embodiments, Cs₂CO₃ as a base and DMAc as the solvent in thesynthesis of the compound of formula VIa. Base Cs₂CO₃ may be substitutedwith K₂CO₃ or KOtBu, each alone or in combination with each other orCs₂CO₃. The compound of formula VIa can be isolated as a solid but canalso be obtained as solution in methyl tert-butyl ether (MtBE).

Synthesis of the acid solvate of the compound of formula II from acompound of formula VIa is as illustrated in Scheme III and exemplifiedin step C of Example 1 below:

According to Scheme III, the compound of formula VIa is dissolved in amixture of an acid R¹—COOH(R′ is as defined hereinabove) and water andheated to about 55-70° C. For example, as described in step C of Example1, the compound of formula VIa is dissolved in acetic acid and water(4:1 AcOH:H₂O) and heated to 67° C. to yield an acetic acid solvate ofthe compound of formula II.

The synthesis of the amide solvate of the compound of formula II fromthe acid solvate of the compound of formula II is as illustrated inScheme IV and exemplified in step C of Example 1:

wherein the compound of formula I is optionally in the form of ahydrate, such as a hexahydrate.

The conversion of the acid solvate of the compound of formula II to theamide solvate of the compound of formula II comprises reslurrying theacid solvate of the compound of formula II in a tertiary amide, such asR³⁰CON(R²)₂ (where R² and R³⁰ are as defined hereinabove) between about20° C.-50° C. For example, as described in step C of Example 1, theacetic acid solvate of the compound of formula II is reslurried in DMFat about 40° C. to yield a DMF solvate of the compound of formula II.

This step of reslurrying of the acid solvate to obtain the DMF solvateof the compound of formula II results in a higher quality product withless starting material and by-products, such as, depletion of thecompound of formula IV to <1 mole % and of p-dimer to <0.1 mole %. Theproduct is stable at 40° C. for about 24 h. This improved processresults in improved filterability of the product. This improved processfurther results in increased yield by about 10%.

A synthesis of the compound of formula I from the amide solvate of thecompound of formula II is as exemplified in step D of Example 1. Theamide solvate of the compound of formula II is taken in an alcohol, suchas, isopropylalcohol/water where the pH is adjusted between about 9 toabout 10.5 by adding a base, such as, NaOH. The solution is heatedbetween about 40° C. to about 80° C. In one embodiment, the DMF solvateof the compound of formula II is treated with isopropylalcohol/water ata temperature of about 80° C. and a pH of about 8-10.2 to result in thecompound of formula I.

IV. EXAMPLES

The invention is further understood by reference to the followingexamples, which are intended to be purely exemplary of certain aspectsof the invention and are not intended to limit the scope.

In the examples below as well as throughout the application, thefollowing abbreviations have the following meanings. If not defined, theterms have their generally accepted meanings.

cm=centimeter

CMCS=chloromethylchlorsulfate

Cs₂CO₃=cesium carbonate

DCM=dichloromethane

DMAc=dimethylacetamide

h=hours

HCl=hydrochloric acid

IPA=isopropylalcohol

mbar=millibar

MeOH=methanol

MtBE=methyl-tert-butyl ether

mol=molar

mL=milliliter

g=gram

mg=milligram

rpm=revolutions per minute

min=minute

mm=millimeter

N=normal

Na₂CO₃=sodium carbonate

NaHCO₃=sodium bicarbonate

NaOH=sodium hydroxide

NMP=N-methylpyrrolidinone

NMR=nuclear magnetic resonance

PDP=di-t-butylphosphate

PTC=phase transfer catalyst

TBAHS=tetrabutylamoniumhydrogehsulfate

v/v=volume/volume

° C.=degree Celsius

POCl₃=Phosphorus oxychloride

Example 1 A. Preparation of di-tert-butyl chloromethyl phosphateProcedure I Preparation of stabilized di-tert-butyl chloromethylphosphate

Preparation of di-tert-butyl chloromethyl phosphate, has been describedin the literature, such as in Mantyla, et al. Tetrahedron Letters, 43(2002), 3793-3794 and Chadwick, et al. U.S. 2006/0047135. We have foundimprovements to these processes whereby the yield is increased, withhigh purity, and the di-tert-butyl chloromethyl phosphate is stabilizedvia exposure to an amide. The specific example below illustrates thisaspect of the invention.

Surprisingly, it was found that di-tert-butyl chloromethyl phosphatecould be synthesized in excellent yield (>90%) and purity (>99%) bydosing 2.5 eq. CMCS to a two phase mixture of PDP and phase transfercatalyst TBAHS in DCM/water and adjusting pH value to 8 at the same timeby addition of 20% aqueous NaOH. Additionally it was found, that thestability of di-text-butyl chloromethyl phosphate was tremendouslyenhanced and no auto catalytic decomposition behaviour was observed bypreparation of a 30 w % solution in dimethylacetamide (DMAc).

Description of the Process:

Di-tert-butyl chloromethyl phosphate was synthesized using a TBAHS phasetransfer catalysed reaction of PDP in DCM/H₂O with 2.5 eq. CMCS at 18°C. The pH was monitored and adjusted to 8 by addition of 20% aqueousNaOH.

The DCM was removed at 20° C. and a pH>7 at reduced pressure (recyclingof DCM). To the crude di-tert-butyl chloromethyl phosphate was addedMtBE, and the TBASHS was removed by washing the MtBE layer with 2%aqueous bicarbonate solution. To stabilize the di-tert-butylchloromethyl phosphate, DMAc was added, and then the MtBE was distilledoff. The yield was >90% based on PDP starting material. The purity ofdi-tert-butyl chloromethyl phosphate in DMAc according to ¹H-NMR>99%

This procedure has at least the following advantages: 1) a liquid base(aqueous NaOH) instead of an excess of solid base such as NaHCO₃,Na₂HPO₄ or Na₂CO₃ can be used; 2) the reaction can be performed in amore concentrated state because an excess of less soluble bicarbonatesor phosphates are replaced by soluble chlorides, thereby minimizingvolume requirements and reaction time while improving yield. Also, thestability of the di-tert-butyl chloromethyl phosphate is enhanced, up to40° C., by preparation of, for example, a 30 w % solution in DMAc.

In a specific example, 56.3 g of PDP (1.0 mol equivalent: 91.2 w %) wasmixed with 3.53 g of TBAHS (0.05 eq.), 60 g of water and 300 g of DCM.At room temperature, 86.6 g of CMCS (2.5 eq.) was dosed to the reactionmixture over 4 h. During the dosing of CMCS, the pH was adjusted to 8 byaddition of 227 g of 20% aqueous NaOH. The resulting two phase reactionmixture was stirred overnight at 20° C. The DCM was distilled off at 20°C. under reduced pressure (500→300 mbar) from the two phase mixture.After addition of 200 mL MtBE to the residue, layers were separated. Thewater layer was discarded and the organic layer was washed once with 300mL 2% aqueous NaHCO₃ to remove phase transfer catalyst. After additionof 90 mL DMAc, MtBE was distilled off at 40° C. and reduced pressure.Liquid nitrogen was bubbled through the resulting mixture for 1 hour toremove traces of DCM and MtBE. The di-tert-butyl chloromethyl phosphate(128 g of an oil) was obtained and analysed by ¹H-NMR, showing a yieldof di-tert-butyl chloromethyl phosphate of 90.7%. (di-tert-butylchloromethyl phosphate: 36.4%; DMAc: 63.4 w %; DCM: 0.03 w %; MtBE: 0.01w %; PTC: 0.01 w %; Water: 0.6 w %).

Additional advantages include: 1) DCM can be recycled, 2) PTC can beremoved in a single extraction, 3) only 5 mol % of PTC is needed, and 4)all but minute traces of DCM and MtBE are remove by bubbling N₂.

B. Preparation of Compound of Formula VIa in MtBE

Description of Synthesis of Compound of Formula VIa:

Cesium carbonate, 27.3 g (1.2 eq.), 185 g of N,N-Dimethylacetamide, 33.5g of compound of formula IV (1 eq., 70 mmol) and 74 g of 30.6 w %di-tert-butyl chloromethyl phosphate (1.25 eq.) in DMAc were charged andstirred at 40° C. overnight. A beige suspension resulted. The suspensionwas cooled to room temperature and 118 mL each of MTBE and water wereadded. The phases were separated and the aqueous layer washed with 94 mLMTBE. The organic layers were each washed with 94 mL water. The organiclayers yielded 187.9 g of compound VIa in MTBE, (yield 74% based on 70mmol compound of formula IV).

C. Preparation of Compound of Formula IIa (Amide Solvate)

Description:

Acetic acid (168.6 g, 160.6 mL) was combined with an equal amount ofwater and the mixture heated to 67° C. The compound of formula VIa (187g, 170 mL) of the solution in MTBE obtained as described above was addedto the aqueous acetic acid. Most of the MTBE was distilled off atatmospheric pressure and the resulting solution stirred for 2 h,resulting in a yellow suspension. The remaining MTBE was distilled offat 300 mbar and the suspension cooled to 20° C. and filtered to give anoff-white solid. The filter cake was washed with cold acetone (2×160 mL)and dried overnight at 30° C. to yield 29.8 g of the compound of formulaII as an acetic acid solvate.

A suspension of 29.8 g of the compound of formula II as an acetic acidsolvate at or about 1:1 stoichiometry) and 150 mL DMF were heated to 50°C. and stirred for 2 h. The suspension was then cooled to roomtemperature and filtered. The filter cake was washed three times with 98mL of MTBE and dried under vacuum overnight at 30° C. The amide solvateof the compound of formula II (26.7 g) was obtained (yield 55% based on70 mmol of compound IV).

D. Preparation of a Hexahydrate of the Compound of Formula I

The amide solvate (10 g, 15 mmol) was suspended in 100 mL water andstirred for 1 hour. Subsequently, 50 mL IPA were added and the pH wasadjusted from 3.3 to 8.5 by addition of 31.9 g of 1M NaOH. The reactionmixture was heated to 82° C., stirred for 1 hour, filtered through a 10micron filter, cooled to 20° C., and stirred over night. The resultingsuspension was filtered and the filter cake washed twice with 40 mLacetone and dried under vacuum over night at 40° C. to give 8.6 g of ahexahydrate of the compound of formula I (77% yield).

What is claimed is:
 1. A composition comprising di-tert-butylchloromethyl phosphate:

and an amide, wherein the amide is of formula R²⁰CON(R²¹)₂ R²⁰ isselected from hydrogen or optionally substituted alkyl; and each R²¹ isindependently hydrogen or optionally substituted alkyl, or both of R²¹and the nitrogen with which they are attached form a 4 to 6 memberedaliphatic ring; or R²⁰ and one of the R²¹ join together with the carbonand nitrogen to which they are attached, respectively, to form a 4 to 6membered nitrogen containing ring, and the other R²¹ is hydrogen oroptionally substituted alkyl; and the optional substituents are selectedfrom alkyl, halo, haloalkyl, nitroso, and cyano.
 2. The composition ofclaim 1, wherein the amide is a solvent.
 3. The composition of claim 2,wherein the amide is a tertiary amide.
 4. The composition of claim 3wherein the tertiary amide is N,N-dimethylacetamide (DMAc).